On February 2007, the Journal of Biotechnology reported work accomplished by Prof. Sun Bing’s group at Antibody Research Center, SIBCB, SIBS, CAS, in cooperation with Institut Pasteur of Shanghai, CAS. This work was about monoclonal antibody generation against non-dominant epitopes of HBV e Ag by a novel antigen-antibody co-immunization method.
Most of monoclonal antibodies raised against HBV e proteins interact with immuno-dominant epitopes. In order to raise antibodies against non-dominant epitopes of HBV e protein, in this study, mice were immunized with both recombinant HBeAg (rHBeAg) and an anti-HBeAg antibody recognizing the dominant antigenic epitope of HBeAg. With this strategy, they successfully selected two monoclonal antibodies binding to recombinant HBeAg with high affinity. The epitope mapping assay determined that the monoclonal antibodies recognize the N-terminal and C-terminal of HBeAg respectively. Further experiment showed that these two antibodies could form a pair-Abs that is used in detecting native HBeAg from the sera of HBV patients. This work is useful for the deeper understanding of the antibody response against e Ag and construction of new detection kit for HBV e Ag.
This work was supported by the Science and Technology Commission of Shanghai Municipality, the National Science Foundation of China and E-institutes of Shanghai Universities Immunology Division.
“Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen–antibody co-immunization”, Journal of Biotechnology 129 (2007) Pages 620–627
Ling Chen Yan a,1, Cai Ying Jing a,1, Chao Feng Huanga, Yong Yong Ji a, Gang Yao a, Xing Feng Cai a, Bing Suna,b,c,
a Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences
b Institut Pasteur of Shanghai, Chinese Academy of Sciences
c E-institutes of Shanghai Universities Immunology Division, PR China